3X Genetics
3X-TGT · Targeted long-read sequencing

Targeted sequencing.
Molecule by molecule.

Measure repeat length, sequence and native methylation together. 3X Genetics brings a detailed view of complex genomic regions to your research.

Explore our services How 3X-TGT works

Research services · Leominster, Massachusetts

A CLOSER LOOK AT YOUR TARGET3X-TGT
One target, many individual DNA moleculesSchematic showing different repeat lengths on individual molecules, a repeat interruption, and methylation marks on flanking DNA. Illustrative only, not sample results.Flanking DNARepeat regionEach row represents one DNA molecule
Repeat lengthSequenceNative methylation

Illustrative molecular view. Not sample results.

EXPLORE THE DETAIL
Research services

Start with your scientific question.

Choose targeted depth, broader genomic coverage or repeat sizing for limited DNA.

1 / TARGETED

3X-TGT sequencing

Characterize repeat expansions and custom genomic targets with native DNA, one molecule at a time.

Explore targeted sequencing
2 / GENOME-WIDE

Long-read genome

Explore sequence, structural variation and methylation with whole-genome and adaptive sequencing options.

Compare genome options
3 / LOWER INPUT

PCR repeat sizing

Screen for expansions or sequence amplified repeats when your project has smaller amounts of DNA.

View PCR services
The science behind 3X-TGT

A repeat has a length.
A sample has a distribution.

Repeat lengths can vary between cells. Reading individual molecules reveals that variation alongside sequence and methylation.

1

Enrich the target

Focus sequencing on the genomic region of interest.

2

Read native DNA

Sequence individual molecules without amplification.

3

Explore the result

Review the locus report, data workbook and alignments.

Explore the technology
Example / DMPK repeat distributionRepeat lengths across individual DNA moleculesDistribution of repeat lengths from the expanded allele in the existing DM1 blood-sample example. Each bar groups molecules by repeat length.020molecules readSomatic expansion across cells3X-TGT deep read05001,0001,500CTG repeats per DNA molecule
Repeat lengths from the expanded allele of a DM1 blood sample. Each bar groups molecules by repeat length.
Who we help

Find the information you need.

Researchers

Plan a study with single-molecule data and research reports.

Research services

Trial sponsors

Define a genetic characterization program for a trial or registry.

Study support

Clinicians

Follow DMPK and TCF4 test development and availability.

Clinical test status

Families & communities

Understand DM1 inheritance and find information and support.

Community resources
Start a conversation

What would you like to measure?

Tell us your target, sample type and study size. Our team can help you choose a service and plan the next step.

Discuss your project